wine derived l plantarum strains  (ATCC)

Journal: bioRxiv

Article Title: A DltE–DltD–DltX interaction network regulates lipoteichoic acid D-alanylation in Lactiplantibacillus plantarum and symbiotic drosophila growth promotion

doi: 10.64898/2026.03.19.713020

Figure Lengend Snippet: A, Schematic representation of the Lp Dlt machinery. DltA transfers D-alanine (D-Ala; with a “+” sign) onto the phosphopantetheinyl arm of DltC, which interacts with the MBOAT protein DltB. DltD and DltX are involved in the extracellular steps of the pathway. Their roles are discussed in the paper. DltE removes D-Ala from LTA and may function in coordination with other Dlt components. B, Amount of D-Ala released from whole cells of NC8 (WT) and derivative mutants by alkaline hydrolysis and quantified by HPLC after derivatization with Marfey’s reagent. Each value represents a single analysis made on a independent experiment. The data are expressed in % of D-Ala compared to the average value of WT (100%). C, Larval longitudinal length after inoculation with strains Lp NC8 , Δ dltX , Δ dltD , DltX Δc-term and DltD 6M . Larvae were collected 6 days after association and measured as described in the Methods section. Purple asterisks illustrate statistically significant difference with Lp NC8 larval size; ****: p<0.0001. Center values in the graph represent means and error bars represent 95% CI. Representative graph from one out of three independent experiments. D, Bacterial load of Lp NC8 (n=3), Δ dltX (n=3), Δ dltD (n=3), DltX Δc-term (n=3) and DltD 6M (n=3) strains recovered from larvae associated with 10 8 CFUs after 6 days association. The bars in the graph represent mean and 95% CI. A representative graph from one out of three independent experiments is shown.

Article Snippet: The 5ʹ- and 3ʹ-terminal regions of dltD region were PCR-amplified with Q5 High-Fidelity 2X Master Mix (NEB) from L. plantarum NC8 chromosomal DNA using primers OL013/OL14 and OL17/OL18.

Techniques: Derivatization

Journal: Food Science & Nutrition

Article Title: Immune‐Enhancing Effects of IBP35 , a Combination of Lactiplantibacillus plantarum and Ligilactobacillus salivarius , in Macrophages and Cyclophosphamide‐Induced Immunosuppressed Mice

doi: 10.1002/fsn3.71831

Figure Lengend Snippet: Strain‐specific immune activation and enhanced activity of the combined formulation in RAW 264.7 macrophages. (A) Heat‐killed L. plantarum IDCC 3501 and L. salivarius IDCC 3551 significantly increased NO production compared with the commercial strain L. rhamnosus GG. (B, C) IDCC 3501 and IDCC 3551 exhibited the highest NO‐inducing activity among their respective species, indicating strain‐specific immune potency. (D, E) The combined formulation (IBP35) further enhanced NO production across all tested concentrations, indicating enhanced activity relative to the individual strains under the tested conditions. Data are presented as the mean ± SD (three independent experiments). Statistical analysis was performed using one‐way ANOVA with Dunnett's post hoc test. Statistical significance was set at p < 0.05. # p < 0.05, ## p < 0.01, ### p < 0.001.

Article Snippet: L. plantarum IDCC 3501 and L. salivarius IDCC 3551 were obtained from the culture collection of Ildong Bioscience Co. Ltd. (Republic of Korea).

Techniques: Activation Assay, Activity Assay, Formulation